Figure EV2. Degranulation of CD56^dimCD16⁺ and CD56^brightCD16⁺ NK cells in response to the MICA-129Met and the MICA-129Val isoforms.

1. Purified IL-2-stimulated NK cells (100 U/ml for 4 days) were cultured for 2 h on plates coated with MICA-129Met-Fc, MICA-129Val-Fc, or OVA-Fc proteins (10 μg/ml) before flow cytometry. The NK-cell populations were gated as illustrated in the upper panel (CD56^dimCD16⁺, CD56^brightCD16⁺, CD56^brightCD16⁻), and CD107a expression was determined as displayed in the lower panel (red: CD107a, black: isotype control). The specific MFI (MFI CD107a minus MFI isotype control) and the percentage of CD107a⁺ cells are indicated.
2. A summary (means + SD) of 5 experiments is shown. The data were analyzed by t-test, and the P-values of significant differences are indicated.