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. 2015 Oct 19;7(11):1480–1502. doi: 10.15252/emmm.201505246

Figure EV4. Down-regulation of NGK2D on CD56dimCD16+, CD56brightCD16+, and CD56brightCD16 NK cells in response to the MICA-129Met and the MICA-129Val isoforms.

Figure EV4

  1. Purified IL-2-stiumlated NK cells (100 U/ml for 4 days) were co-cultured with L-con, L-MICA-129Met, or L-MICA-129Val clones for 4 h. Three NK-cell populations (CD56dimCD16+, CD56brightCD16+, CD56brightCD16) were gated as illustrated in the upper panel. The NKG2D expression on these NK-cell populations is displayed in the lower panel, and the MFI of NKG2D and the percentages of NKG2D+ cells are indicated.
  2. A summary (means + SD) of NKG2D expression on the three NK-cell populations 4 and 24 h after co-culture with L-con (= 3), L-MICA-129Met (= 17), and L-MICA-129Val clones (= 18) is displayed. The NKG2D expression (MFI) at the beginning (0 h) was set to 100%. The average reduction of NKG2D on NK cells in response to L-MICA-129Met compared to L-MICA-129Val clones at 4 and 24 h (%-points) is indicated in the panels. The differences were analyzed by repeated measures ANOVA, and P-values are indicated.