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. Author manuscript; available in PMC: 2015 Nov 14.
Published in final edited form as: Cell Signal. 2013 Jan 16;25(4):1027–1035. doi: 10.1016/j.cellsig.2013.01.008

Figure 7.

Figure 7

Syncytin-1 overexpression in CHO cells promoted DNA synthesis. Representative flowcytometry diagrams shows that compared to control cells transfected with pEGFP-N1 (A), the syncytin-1 overexpression group transfected with pEGFP-N1-syncytin1 (B) contains increased S phase at 72 hour post-transfection. (C) BrdU incorporation assay was performed at 72 hour post-transfection in CHO cells transfected with control plasmid pEGFP-N1 (left panel) or syncytin-1 expression plasmid pEGFP-N1-syncytin-1 (right panel). Nuclei were visualized by hemotoxylin counterstaining. The dark brown color staining indicated BrdU-positive nuclei. One typical positive cell is marked by an arrow. (D) Counting of BrdU-positive and total cells showed a significantly increased percentage of BrdU-positive cells in the syncytin-1 overexpression group compared to the control group. (**, p < 0.01)