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. Author manuscript; available in PMC: 2016 Nov 17.
Published in final edited form as: Vaccine. 2015 Sep 26;33(46):6332–6339. doi: 10.1016/j.vaccine.2015.09.015

Figure 2. 6CMUNic-KLH elicits greater hapten-specific serum IgG antibody titers and splenic polyclonal hapten-specific B cells in mice.

Figure 2

A) 6CMUNic-KLH elicited higher serum IgG antibody titers than 2CMUNic-KLH, measured at 35 days, a week after the last immunization. The reported p value is based on analysis of log-transformed titers to meet normality requirements. In these mice, B cell analysis was performed by means of splenectomy 14 days after immunization. B) The total number of lymphocytes and B cells was not different in the spleen fragments from mice immunized with either 6CMUNic-KLH or 2CMUNic-KLH. C) Immunization with 6CMUNic-KLH was more effective in inducing GC, IgMhigh, and ASC and swIg hapten-specific B cells. Shown the number of hapten-specific B cells in individual spleen fragments after enrichment. Although B cell analysis was performed using either the 6CMUNic-PE or the 2CMUNic-PE enrichment reagents for each respective immunization group, hapten-specific B cells are reported as CMUNic-specific B cells. Data are mean±SEM, n=6 each group. Statistical symbols: *p<0.05, ***p<0.001, NS= not significant.