Table I.
B cell subset | Analysis Time point | Nicotine-specific IgG titers at 35 days | Vaccine efficacy on serum nicotine | Vaccine efficacy on brain nicotine |
---|---|---|---|---|
IgMhigh | 14 days | r2= 0.23, NS | r2=0.5, p< 0.01 | r2= 0.44, p<0.05 |
swIg | 14 days | r2= 0.1, NS | r2=0.3, p< 0.05 | r2=0.2, p< NS |
GC | 14 days | r2= 0.34, p<0.05 | r2=0.34, p< 0.05 | r2=0.2, p< NS |
ASC | 14 days | r2= 0.2, NS | r2= 0.15, NS | r2= 0.03, p< NS |
Shown the regression coefficient (r2) between numbers of hapten-specific B cells 14 days after the first immunization and vaccine efficacy in mice with 6CMUNic-KLH and 2CMUNic-KLH (n=12 total sample size). Mice were immunized s.c. in alum on days 0, 14 and 28. Serum antibodies and vaccine effects on nicotine distribution were analyzed a week after the last immunization. NS= not significant.