Fig. 4. The RLIP76 N-terminus and R-Ras interaction are required for fibroblast migration.
Primary embryonic fibroblasts derived from RLIP76-null or isogenic wild type mice were transfected as indicated plus GFP. Equal numbers of wild type or RLIP76-null (KO) fibroblasts transfected as shown were seeded onto fibronectin-coated surfaces to form confluent monolayers. A single scratch was made through the monolayer with a pipet tip and GFP-positive cells at the wound margins were imaged over time, from which migration distances were calculated. Average migration distances from the starting point are shown ± SEM. *, p < 0.03; **, p < 0.02 (n=5).
