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. Author manuscript; available in PMC: 2015 Nov 14.
Published in final edited form as: Cell Rep. 2015 Oct 29;13(6):1258–1271. doi: 10.1016/j.celrep.2015.09.064

Figure 1. A majority of Atoh1-lineage neurons reside outside Clarke’s column.

Figure 1

(A) ATOH1 is transiently expressed in the developing neural tube (left, antibody staining). Atoh1-lineage neurons in the caudal rhombic lip develop into neurons of the external cuneate nucleus (ECu) in the medulla (cuneocerebellar tract, CCT, dark grey), and the lateral reticular nucleus (LRt, dark grey). In the spinal cord, it is unknown if Atoh1-lineage neurons contribute to Clarke’s column (CC, marked by Vglut1 or Gdnf, black) or non-CC (dark grey) sources of the dorsal spinocerebellar tract (DSCT). (B) Vglut1 mRNA identifies neurons in the ECu and LRt. (C) A majority of Atoh1-lineage neurons (Atoh1Cre/+; R26RLSL-EGFP, GFP Ab, green) in the ECu and LRt express Vglut1 mRNA (arrows in C, 86.7 ± 1.3% and 91.8 ± 0.2%, respectively, 3 representative sections counted per n=2 medulla from 1 litter, arrowheads for Vglut1+ only cells). (D) Very few Atoh1-lineage neurons colocalize with Vglut1 in CC (0.5 ± 0.1%, 18 representative thoracic 5-13 (T5-13) sections per n=3 spinal cords from 2 litters, arrows for GFP+, arrowheads for Vglut1 mRNA). (E) Atoh1-lineage neurons in the thoracic spinal cord are glutamatergic (82.2 ± 2.2%, 15 representative T5-13 sections per n=3 spinal cords from 2 litters, Vglut2+, arrows). Mean ± SEM shown. Scale bars are 100 μm, except C and insets in D and E are 50 μm. See Figure S1 for characterization of the Atoh1Cre/+ mouse.