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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Biomaterials. 2015 Oct 9;75:58–70. doi: 10.1016/j.biomaterials.2015.10.002

Figure 4. Effect of Pluronic on the gene expression in RAW 264.7 MØs.

Figure 4

(a, b) Cells were exposed to gWIZ™ Luc pDNA with or without P85 in SFM (a) for 2 h using 1 µg pDNA and various concentrations of P85 or (b) for 4 h using different amounts of pDNA (1 µg, 5 µg and 10 µg) and 1.0% w/v P85. (a, b) Cells transfected using genePORTER® 3000 transfection reagent are presented as positive control. Data were acquired using Glomax 20/20 luminometer or M5 Molecular Devices microplate reader. Data are mean ± SEM (n=4), * p<0.05, ** p<0.01, **** p<0.001 n.s. – non significant. Statistical comparisons were made for (a) treated vs. untreated groups or (b) otherwise indicated groups by one-way ANOVA with Bonferroni correction for multiple comparisons.