Table 1.
Composition of experimental diet (AIN-93 modified diet for rodents)
| Ingredient | g/kg diet |
|---|---|
| Cornstarch | 465.692 |
| Casein (>85% protein) | 140.000 |
| extrinized cornstarch (90–94% tetrasaccharides)1 | 155.000 |
| Sucrose | 100.000 |
| Soybean oil (no additives) | 40.000 |
| Fiber2 | 50.000 |
| Mineral mix | 35.000 |
| Vitamin mix | 10.000 |
| L-Cystine | 1.800 |
| Choline bitartrate (41.1% choline)3 | 2.500 |
| Tert-butylhydroquinone | 0.008 |
| u/kg diet | |
| Total energy4 kcal | 3601.0 |
| protein% | 14.1 |
| CHO% | 75.9 |
| fat% | 10.0 |
1
Dyetrose (Dyets, Bethlehem, PA) and Lo-Dex 10 (American Maize, Hammond, IN) meet these specifications. An equivalent product may also be used./
2
Solka-Floc®,200 FCC (FS&.D, St. Louis, MO) or its equivalent is recommended./
3
Based on the molecular weight of the free base./
4
The estimate of caloric content was based on the standard physiological fuel values for protein, fat, and carbohydrate of 4, 9 and 4, respectively.