Effects of deleting corepressor genes MTH1 and STD1 on glucose uptake and ethanol production rates. Wild-type (WT; CEN.PK2-1C) and mth1Δ, std1Δ, and mth1Δ
std1Δ
S. cerevisiae cells were grown in SC (A) or YPD (B) medium with 80 g/liter glucose, and glucose and ethanol levels were detected during growth. (C) Expression levels of HXT genes. The indicated strains were grown in YPD (80 g/liter) medium for 6 h, and the mRNA expression levels of each HXT gene were measured by qRT-PCR and normalized to that of the ACT1 gene. The mRNA levels relative to that of the wild-type control are indicated with standard deviations of the results of three independent experiments. *, P < 0.05 (compared to the wild type).