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. 2015 Nov 13;81(24):8392–8401. doi: 10.1128/AEM.02056-15

FIG 3.

FIG 3

Improvement of glucose uptake rate by overexpressing GCR1. (A) Effects of overexpressing GCR1 and/or GCR2 on the glucose uptake rate. CEN.PK2-1C cells overexpressing GCR1 and GCR2 alone or in combination were grown in SC-Leu-Ura medium with 80 g/liter glucose and monitored for glucose uptake and ethanol production. Cells expressing empty vectors pRS415GPD and pRS416GPD were used as a control. (B) Effects of overexpression of the GCR1 gene on the transcription levels of its target genes. CEN.PK2-1C cells overexpressing GCR1 or harboring pRS416GPD (control) were grown in SC-Ura medium with 80 g/liter glucose for 12 h, and mRNA expression levels of Gcr1 target genes were measured by qRT-PCR and normalized to the ACT1 level. The mRNA levels relative to those of the control are indicated with standard deviations of the results of three independent experiments. *, P < 0.05 (compared to control). (C) Effects of overexpressing GCR1 alone or in combination with HXT7. Cells overexpressing the indicated genes were grown in SC-Trp-Ura medium with 80 g/liter glucose and monitored for glucose uptake and ethanol production. Cells expressing empty vectors (pRS414GPD and pRS416GPD) were used as a control. Mean values of triplicate experiments are shown with error bars indicating standard deviations. *, P < 0.05 (compared to control).