TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| S. cerevisiae CEN.PK2-1C | MATa ura3-52 trp1-289 leu2-3_112 his3Δ1 MAL2-8c SUC2 | EUROSCARFa |
| S. cerevisiae JHY701 | CEN.PK2-1C mth1Δ | This study |
| S. cerevisiae JHY702 | CEN.PK2-1C std1Δ | This study |
| S. cerevisiae JHY703 | CEN.PK2-1C mth1Δ std1Δ | This study |
| S. cerevisiae SP2006 | CEN.PK2-1C pdc1Δ::Pccw12-BtLDH cyb2Δ::Pccw12-BtLDH gpd1Δ::Pccw12-PsjLDH adh1Δ::Pccw12-PsjLDH | 29 |
| S. cerevisiae SP2018 | SP2006 his3Δ::PTDH3-GCR1 | This study |
| S. cerevisiae SP1130 | SP2006 ald6Δ leu2Δ::PTDH3-mhpF pdc6Δ::PTDH3-eutE | 29 |
| S. cerevisiae SP1141 | SP1130 his3Δ::PTDH3-GCR1 | This study |
| Plasmids | ||
| pRS414GPD | CEN/ARS plasmid, PTDH3, TCYC1, TRP1 marker | 30 |
| pRS415GPD | CEN/ARS plasmid PTDH3, TCYC1, LEU2 marker | 30 |
| pRS416GPD | CEN/ARS plasmid, PTDH3, TCYC1, URA3 marker | 30 |
| pUG72 | Plasmid containing loxP-URA3-loxP deletion cassette | EUROSCARF |
| pSH63 | Plasmid containing a gene for Cre-recombinase, TRP1 marker | EUROSCARF |
| pRS414GPD-HXT1 | HXT1 ORF cloned between the BamHI and ClaI sites of pRS414GPD | This study |
| pRS414GPD-HXT2 | HXT2 ORF cloned between the BamHI and ClaI sites of pRS414GPD | This study |
| pRS414GPD-HXT3 | HXT3 ORF cloned between the BamHI and ClaI sites of pRS414GPD | This study |
| pRS414GPD-HXT4 | HXT4 ORF cloned between the BamHI and ClaI sites of pRS414GPD | This study |
| pRS414GPD-HXT7 | HXT7 ORF cloned between the BamHI and XhoI sites of pRS414GPD | This study |
| pRS416GPD-GCR1 | GCR1 ORF cloned between the XbaI and XhoI sites of pRS415GPD | This study |
| pRS415GPD-GCR2 | GCR2 ORF cloned between the XbaI and XhoI sites of pRS416GPD | This study |
| pUC57-URA3 | Plasmid containing HA-URA3-HA deletion cassette | 29 |
| pUC57-URA3–GCR1 | PTDH3-GCR1-TCYC1 cloned into the KpnI site of pUC57-URA3 | This study |
a
EUROSCARF, European Saccharomyces cerevisiae Archive for Functional Analysis.