TABLE 1.
Strains and plasmids used in this study
| Plasmid or strain | Description | Reference or source |
|---|---|---|
| Plasmids | ||
| pRL27 | Tn5-RL27 (Kmr-oriR6K); Kmr | 22 |
| pCR2.1 | TOPO cloning vector | Invitrogen |
| Strains | ||
| Escherichia coli | ||
| TOP10 | General cloning strain | Invitrogen |
| One Shot PIR1 | General cloning strain, pir-116 (used for rescue cloning) | Invitrogen |
| EA145 | 2,4-Diaminopimelic acid auxotroph, RP4 (used for mutagenesis using pRL27) | Gift from A. Buchan, The University of Tennessee |
| Azospirillum brasilense | ||
| Sp7 | Wild-type strain (ATCC 29145) | Laboratory collection |
| AB7001 | Sp7 exoC::Tn5; Kmr | 51 |
| AB104 | Sp7 ΔcheY1::Km; Kmr | 7 |
| BS106 | Sp7 Δ(cheB1-cheR1)::Km; Kmr | 52 |
| phbC | Sp7 Δ(phbC)::Km; Kmr | 43 |
| Sp72002 (flcA) | Sp7 flcA::Tn5; Kmr | 13 |
| EJ1 (rmlD) | Sp7 rmlD::Tn5; Kmr | 53 |
| FAJ310 | Sp7 amtB::Km; Kmr | 54 |
| 7628 (glnB) | Sp7 glnB::Km; Kmr | 42 |
| 7611 (glnZ) | Sp7 glnZ::Ω; Spr Smr | 42 |
| 7148 (ntrC) | Sp7 ntrC::Tn5-148; Kmr | 55 |