TABLE 2.
List of primers and probes used in this study
| Primer or probe | Sequence (5′ to 3′)a | Purposeb | Reference or source |
|---|---|---|---|
| RHO1inlATqMnF | CGG ATG CAG GAG AAA ATC CTA TAC | Amplification of a 134-bp fragment of the 5′ region of inlA | This study |
| RHO2inlATqMnR | CGC TGC CAA ATA CTA ATA TTG CTA CTA G | Amplification of a 134-bp fragment of the 5′ region of inlA | This study |
| inlA proF | TTT TAA AAG GTG GAA TGA CA | Sequencing primer for entire inlA ORF | 24 |
| inlA proR | GAA GCG TTG TAA CTT GGT CTA | Sequencing primer for entire inlA ORF | 24 |
| inlA F1 | CAG GCA GCT ACA ATT ACA CA | Sequencing primer for entire inlA ORF | 24 |
| inlA S1R | GGA CTG ATG TTA CTT ATT TGG T | Sequencing primer for entire inlA ORF | 24 |
| inlA F2 | AAG ATA TAG GCA CAT TGG CGA GTT | Sequencing primer for entire inlA ORF | 24 |
| inlA S2R | CGT ACT GAA ATY CCA KTT AGT TCC | Sequencing primer for entire inlA ORF | 24 |
| inlA seq | GTG GAC GGC AAA GAA ACA AC | Sequencing primer for entire inlA ORF | 24 |
| F inlA R | ATA TAG TCC GAA AAC CAC ATC T | Sequencing primer for entire inlA ORF | 24 |
| RHO8-inlA-TqMn-WT | TAGTGAGAAAAAAACGATATG (reporter, FAM; quencher, MGB) | TaqMan probe for detection of A7 HT | This study |
| RHO9-inlA-TqMn-FS | AGTGAGAAAAAACGATATG (reporter, VIC; quencher, MGB) | TaqMan probe for detection of A6 HT | This study |
| CSM1-inlA-TqMn-Gtrans | ATAGTGAGAAGAAAACGATATG (reporter, NED; quencher, MGB) | TaqMan probe for detection of A2GA4 HT | This study |
a
MGB, minor groove binder; FAM, 6-carboxyfluorescein. VIC and NED are commercially available fluorescent dyes.
b
ORF, open reading frame.