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Time course of hydrolyzing o-chloromandelonitrile mediated by the WT enzyme (BCJ2315) and the MG (I113M/Y199G) double mutant in an ethyl acetate-water (1:9, vol/vol) system. The reaction mixture contained 10 g/liter wet cells, 0.5 M o-chloromandelonitrile, 10% (vol/vol) ethyl acetate, and 90% sodium phosphate buffer (pH 8.0).
