TABLE 2.
PCR primers and probes used in the study
| Name | Sequence (5′→3′) | Oligonucleotide type (label) | Specificity | Reference |
|---|---|---|---|---|
| BACT365_281_337F | CGTCGTCTAATATCCCATAACATTG | PCR primer | Bacteroidetes bacteria HOT 365 and HOT 281 | This study |
| LACH500_50F | GCAAGTTGAACGAGAAGTTCC | PCR primer | Lachnospiraceae bacterium HOT 500 | This study |
| CLOST093_312F | CCTCTGTCTACGGGATAACA | PCR primer | Clostridiales bacterium HOT 093 | This study |
| 519R | GWATTACCGCGGCKGCTG | PCR primer | Domain Bacteria | 20 |
| 27FYM | AGAGTTTGATYMTGGCTCAG | PCR primer | Domain Bacteria | 28 |
| Lach500_786 | CTATAGGCCAGCACCTAGC | Probe (Cy5) | Lachnospiraceae bacterium HOT 500 | This study |
| Lach500_1206 | CTTCGCTTCCCTTTGTATCC | Probe (Cy5; DIG) | Lachnospiraceae bacterium HOT 500 | This study |
| Eub338 | GCTGCCTCCCGTAGGAGT | Probe (Cy3) | Domain Bacteria | 29 |
| NonEub | TGAGGATGCCCTCCGTCG | Probe (FITC) | Nonsense probe | 29 |