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HPLC and mass spectrum detection of the intermediate metabolites. (A to D) HPLC and positive-ion mass spectra of the substrate MEA (A), of the metabolite product (MEHQ) by strain MEA3-1Mut (B), of the metabolite product (4-OH-MEA) by strain MEA3-1Mut (C), and of the metabolite product (3-OH-MEHQ) by strain MEA3-1 (D).
