TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Characteristic(s)a | Source or reference |
|---|---|---|
| Strains | ||
| Sphingobium sp. MEA3-1 | Wild-type MEA degrader, pMEA01–pMEA07, Smr | 2 |
| Sphingobium sp. MEA3-1Mut | Derivative of MEA3-1, deletion within pMEA02 | This study |
| P. putida KT2440 | A model strain for aromatic catabolism, Cmr Ampr | 31 |
| E. coli DH5α | Host strain for cloning vectors | TaKaRa |
| E. coli HB101(pRK600) | Conjugation helper strain, Cmr | 33 |
| Plasmids | ||
| pBBR1MCS-2 | Broad-host-range cloning vector, Kmr | 30 |
| pBBR-meaA | pBBR1MCS-5 derivative carrying meaA, Kmr | This study |
| pBBR-meaBA | pBBR1MCS-5 derivative carrying meaBA, Kmr | This study |
| pBBR-meaBA-orf1-4 | pBBR1MCS-5 derivative carrying 7,479 bp | This study |
| pET29a-meaBAT7 | pET29a(+) derivative carrying meaBA, Kmr | This study |
| pMD19-T | T-A cloning vectors, Ampr | TaKaRa |
a
Smr, streptomycin resistant; Cmr, chloramphenicol resistant; Ampr, ampicillin resistant; Kmr, kanamycin resistant.