TABLE 3.
Substrate | Result witha: |
|
---|---|---|
MEA3-1 | MEA3-1Mut | |
MEA | + | * |
DEA | + | * |
DMA | + | * |
Aniline | − | − |
2,3-DMA | − | − |
2,4-DMA | − | − |
2-MA | + | * |
4-OH-DMA | + | − |
DMHQ | + | − |
Toluene | − | − |
O-Xylene | − | − |
Phenol | + | + |
Catechol | + | + |
Hydroxyquinol | + | + |
Hydroquinone | + | + |
2-MP | + | * |
2,6-DMP | + | * |
3,5-DMP | + | * |
3,4-DMP | − | − |
+, positive for strain growth and substrates were degraded; −, negative for strain growth and substrates were not degraded; *, negative for strain growth and substrates were transformed to other compounds. The biomass was measured at 600 nm (OD600) using a Shimadzu UV-visible spectrophotometer. The mineralization or cometabolic transformation of these aromatic compounds was measured using HPLC, all treatments were performed in triplicate, and control experiments without inoculation and without substrate were performed under the same conditions.