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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Mol Cancer Res. 2015 Jul 29;13(11):1509–1519. doi: 10.1158/1541-7786.MCR-15-0204

Figure 1.

Figure 1

Inhibition of pancreatic cancer growth by calcipotriol correlates with levels of VDR expression and autocrine Wnt/β-catenin signaling. (A) Western blot for VDR and tubulin (loading control) and mean Wnt reporter activity as indicated by BAR/fuBAR ratios and measured by dual luciferase assays 48 hours after co-transfection with control Renilla and either BAR-luciferase or fuBAR-luciferase reporter constructs in indicated PDAC cell lines (n=3 biological replicates). (B) MTT growth assays (measured at 72 hours) and (C) soft agar colony formation at indicated concentrations of calcipotriol. Representative images of AsPC-1 soft agar colony formation are also shown. Data are normalized to vehicle only and reported as mean ± standard deviation (SD) with one representative experiment of 3-5 biological repeats shown. *P<0.05, **P<0.01, ***P<0.001.