Flow diagram of specimens in the training and validation cohort. Specimen cohorts for all urine samples assessed for TMPRSS2:ERG (T2:ERG) and PCA3 are shown. Samples excluded from various analyses (indicated by legend and described in the text) are indicated in red. Samples assessed using version 2 or version 3 (final assay) T2:ERG transcription-mediated amplification assays are indicated.
AS= active surveillance; EDRN = Early Detection Research Network; PCPTrc = Prostate Cancer Prevention Trial risk calculator; PCPThg = PCPT high grade cancer risk calculator; PSA = prostate-specific antigen; ROC = receiver operating characteristic; T2:ERG = TMPRSS2:ERG;UMHS = University of Michigan Health System; v3 = version 3.