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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Dev Neurobiol. 2015 Jun 1;76(1):93–106. doi: 10.1002/dneu.22301

Figure 4. dNab2 is required in neurons for mushroom body development.

Figure 4

A, Merged projections (projection) and single 1.5 µm transverse slices (midline) of FasII-positive α/β-lobes of Dcr2 control flies (top, genotype: elav-Gal4/+ or Y; UAS-dcr2/+) or flies with RNAi knockdown of dNab2 in all neurons (bottom, genotype: elav-Gal4/+ or Y; UAS-dcr2/UAS-dNab2RNAi). dNab2-depleted neurons show evidence of mushroom body fusion (arrow). B, Quantification of missing/thin α-lobes or missing/over-extending β-lobes as observed in A (Drc2 alone: males n=20, females n=14; Dcr2+dNab2-RNAi: males n=15, females n=14). All flies contain elav-Gal4>UAS-Dcr2. C, qPCR analysis of dNab2 levels in the indicated genotypes (as in A). Data represents an average of three biological replicates of ~50 adult fly heads for each genotype. Precise excision control (dNab2pex41/dNab2pex41) flies and dNab2ex3 homozygous null flies (dNab2ex3/dNab2ex3) were used as positive and negative controls, respectively. Statistical significance values via two-tailed independent t-test are shown between indicated samples.