Table 1.
Examples for application of marker-assisted selection (MAS) and marker-assisted backcrossing (MABB) in rice.
| Trait | Gene(s)/QTL(s) | Marker(s) used | Technique used | Application | Reference |
|---|---|---|---|---|---|
| Blast resistance | Pi1, Piz-5, Pita | RFLP | MAS | Pyramiding of three near isogenic lines (C101LAC, C101A51 and C101PKT) for blast resistance in into a single cultivar Co-39, each carrying the major genes Pi1, Piz-5 and Pita, respectively | Hittalmani et al., 2000 |
| Blast resistance | Pi1 | SSR and ISSR | MAS | Applied for backcross breeding of variety (Zhenshan 97A) | Liu et al., 2002b |
| Bacterial blight Resistance + Blast resistance | Xa21, Piz | SSR | MAS | Functional for pyramiding of target traits | Narayanan et al., 2002 |
| Blast resistance | Pid1, Pib, Pita and Pi2 | SSR | MAS | Pid1, Pib and Pita genes were introduced into G46B cultivar, while Pi2 Zhenshan97B cultivars of rice | Chen et al., 2004 |
| Blast resistance | Pi-z | SSR | MAS | Closely linked with Pi-z locus has been successfully used for selection of blast resistance in a wide array of rice germplasm | Fjellstrom et al., 2006 |
| Blast resistance + Bacterial blight resistance + Sheath blight resistance | Xa13, Xa21, Pi54, qSBR11 | SSR [for blast resistance (Xa13 and Xa21), for bacterial blight resistance (Pi54), and Sheath blight resistance (qSBR11)] | MAS | MAS-assisted transfer of genes conferring the resistance toward three different diseases in rice | Singh et al., 2012a |
| Blast resistance + Bacterial blight resistance | Pi-genes, Xa5 | SSR | MAS | Near-isogenic lines (NILs) derived from two blast resistant crosses (RD6 × P0489 and RD6 × Jao Hom Nin) were pyramided with IR62266 (xa5), to transfer bacterial leaf blight resistance to RD6 lines | Pinta et al., 2013 |
| Blast resistance | Pi-ta | Gene specific marker | MAS | Existence of the Pi-ta gene in 141 rice germplasm has been successfully determined, but the results were more articulated when Pi-ta gene was introduced through advanced breeding lines | Wang et al., 2007 |
| Submergence tolerance + Brown planthopper resistance + Blast resistance + Bacterial blight resistance | chr9 QTL, Xa21, Bph and QTLs blast, and quality loci | SSR and STS | MABB | MABB confirmed the transfer of gene and QTL for into cultivar KDML105 | Toojinda et al., 2005 |
| Blast resistance | Pi1, Pi2, Pi33 | SSR | MABB | Introgressed into Jin23B cultivar through MABB | Chen et al., 2008 |
| Blast resistance + Bacterial blight | Pi1, Pi2, Xa23 | SSR [For blast resistance (Pi1, Pi2), for bacterial blight resistance (Xa23) | MABB | Sucessfully applied for breeding the variety (Rongfeng B) | Fu et al., 2012 |
| Blast resistance | Piz-5, Pi54 | SSR | MABB | Combination of blast resistance gene from donor lines (C101A51 and Tetep) into cultivar PRR78 to develop Pusa1602 (PRR78 + Piz5) and Pusa1603 (PRR78 + Pi54), respectively | Singh et al., 2012b |
| Blast resistance | Pi-9(t) | pB8 | MABB | MABB applied to introgress the cultivar Luhui17 | Wen and Gao, 2011 |
| Blast resistance | Pi-1, Pi-z | SSR | MABB | Pyramiding of Pi-1 and Piz-5 genes into introduced PRR78 cultivars | Gouda et al., 2013 |