Table 2.
LN- and FN-binding proteins identified by MALDI-TOF-MS and bioinformatics analysis.
| Spot no.a | Identified protein | Accession no. | Theoretical pI/MWb | Experimental pI/MW | Mascot score | Coverage (%) | PSORT predictionc | Reported surface protein |
|---|---|---|---|---|---|---|---|---|
| 1 | Amino acid ABC transporter permease | WP_012775143.1 | 4.55/53192 | 4.3/53000 | 105 | 25 | Unknown | |
| 2, 5, 23 | enolase | YP_001201070.1 | 4.66/47066 | 4.7/50000 | 340 | 69 | Cytoplasmic | Yes |
| 3 | Oligopeptide-binding protein OppA precursor | YP_003025672.1 | 4.89/65672 | 4.8/66000 | 211 | 49 | Unknown | |
| 4 | Elongation factor Tu | YP_001197896.1 | 4.87/44755 | 4.9/47000 | 164 | 40 | Cytoplasmic | |
| 6 | Phosphoglycerate kinase | WP_029998367.1 | 4.86/42053 | 5.0/42000 | 208 | 62 | Cytoplasmic | |
| 7,8 | Pyruvate kinase | WP_029186723.1 | 5.24/54836 | 5.3/56000 | 290 | 58 | Cytoplasmic | |
| 9,10 | Glutamate dehydrogenase | ACG49984.1 | 5.43/48811 | 5.6/46000 | 162 | 37 | Cytoplasmic | Yes |
| 11 | Inosine 5′-monophosphate dehydrogenase | WP_023371888.1 | 5.69/52748 | 6.1/56000 | 244 | 45 | Cytoplasmic | |
| 12 | Glyceraldehyde-3-phosphate dehydrogenase | AAZ78247.1 | 5.58/35883 | 5.6/37000 | 130 | 40 | Cytoplasmic | Yes |
| 13 | 6-phosphofructokinase | WP_029188183.1 | 5.32/35360 | 5.9/36000 | 191 | 55 | Cytoplasmic | |
| 14 | Pyrophosphatase | WP_009910564.1 | 4.59/33367 | 4.5/35000 | 321 | 54 | Cytoplasmic | |
| 15, 16, 17, 20 | Fructose-bisphosphate aldolase | WP_029185467.1 | 4.90/31157 | 5.0/31000 | 134 | 49 | Cytoplasmic | |
| 18, 19 | Lactate dehydrogenase | WP_029999076.1 | 5.14/35390 | 5.2/36000 | 276 | 48 | Cytoplasmic | |
| 21, 22 | 3-ketoacyl-ACP reductase | WP_032511883.1 | 5.29/25533 | 5.5/25000 | 213 | 84 | Cytoplasmic | |
| 24 | Cell wall surface anchor protein | WP_012027978.1 | 5.75/32143 | 5.1/31000 | 107 | 36 | Unknown | |
| 1′ | Oligopeptide-binding protein OppA precursor | YP_003025672.1 | 4.89/65672 | 4.8/66000 | 253 | 35 | Unknown | |
| 2′, 6′ | enolase | YP_001201070.1 | 4.66/47066 | 4.7/50000 | 256 | 37 | Cytoplasmic | Yes |
| 3′, 4′, 5′, 7′ | Elongation factor Tu | YP_001197896.1 | 4.87/44755 | 4.9/47000 | 228 | 46 | Cytoplasmic | |
| 8′, 9′ | Fructose-bisphosphate aldolase | WP_029185467.1 | 4.90/31157 | 5.0/31000 | 178 | 42 | Cytoplasmic | |
| 10′ | Lactate dehydrogenase | WP_029999076.1 | 5.14/35390 | 5.2/36000 | 276 | 48 | Cytoplasmic |
a
Spot numbers correspond to those indicated in Figure 1.
b
Theoretical pI and MW were calculated by Compute pI/Mw server (http://web.expasy.org/compute_pi/).
c
Subcellular location of identified proteins is predicted by PSORTb (http://www.psort.org/psortb/index.html).