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. 2015 Nov 16;5:16323. doi: 10.1038/srep16323

Figure 1. Generation of haFGF transgenic silkworm and the expression analysis of recombinant proteins.

Figure 1

(A) Structure map of the transgenic vector. (B) Fluorescent images of the transgenic silkworm in egg (a,b) and moth (c,d) stages, the scale bar represents 2 mm. (C) Immunohistochemical analysis of MSG cross sections of transgenic silkworm (a–c) and non-transgenic silkworm (d–f). The green fluorescence represents the immunoblot signals of haFGF proteins; the DAPI stained by blue fluorescence represents the cell nucleus. Scale bar represents 500 μm. (D) Immunohistochemical analysis of raw silk cross sections of transgenic silkworm (a,b) and non-transgenic silkworm (c,d). Scale bar represents 10 μm. (E) SDS–PAGE analysis of the cocoon proteins from ten different transgenic lines; the percentages represent the haFGF content of each line in the total cocoon extracts. (F) Western blot analysis of the haFGF in cocoon extracts from ten different transgenic lines.