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. 2015 Nov 16;5:16323. doi: 10.1038/srep16323

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(A) The procedure of the PIG transposase mediated transposon remobilization strategy. (B) Large-scale analysis of haFGF expression patterns in individuals of the offspring after hybridization and segregation by ELISA. (C) SDS–PAGE analysis of the haFGF proteins in cocoons from typical strains after hybridization with the jumpstarter. The asterisk indicates haFGF proteins. (D) Western blot analysis of haFGF proteins in the cocoons of typical strains after hybridization with the jumpstarter. The numbers represent the increased folds of haFGF expression levels in the remobilized mutants compared to that of the A11 line. (E) Genetic analysis of the insertion loci of the mutant strains afte r hybridization with the jumpstarter.