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. 2015 Nov 16;5:16567. doi: 10.1038/srep16567

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Copyright © 2015, Macmillan Publishers Limited

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(A) Heat map of gene expression in negative control (NC), Ring1B knockdown (KD), and Dzip3 KD mES cells in biological duplicate RNA-seq samples. Blue and red indicate down- and upregulated genes, respectively. (B) Venn diagram representing the genes upregulated by Ring1B KD and Dzip3 KD cells. The numbers represent the numbers of genes upregulated (>1.5 or >2.0 fold) by KD of either one of the two proteins or by KD of both proteins. (C) Gene ontology (GO) analysis of genes upregulated by both Ring1B KD and Dzip3 KD. (D) RT-qPCR results for major developmental genes (Neurod1, Rhox6, Cdh2, Neurog1). Error bars, standard deviation.