Figure 4. Involvement of cytoskeletal components in neurite outgrowth during microheating.

Summary of the elongation lengths of neurites after 60 s heat treatment in the absence of chemical agents (Control), and in the presence of 10 nM vinblastine (Vinb), 30 μg·mL⁻¹ colchicine (Col), 10 μM nocodazole (Noco), 20 μM taxol, 10 μM cytochalasin D (CytoD), 10 μM latrunculin B (LatB), 30 μg·mL⁻¹ colchicine and 10 μM cytochalasin D (Col + CytoD), 30 μg·mL⁻¹ colchicine and 10 μM latrunculin B (Col + LatB), 25 μM ciliobrevin D (CilioD), 25 μM blebbistatin (Blebbi), 20 μM ML-7, or 10 μM Y-27632. Bars indicate average values. The laser power was 11 mW, the ΔT was 4.9 ± 0.4 °C (means ± s.d.), and the T₀ was 36 °C. Elongation lengths were compared by one-way ANOVA with Tukey-Kramer tests (**p < 0.01, ***p < 0.001; NS, not significant).