Figure 3. siRNA knockdown of CST increased ER stress-induced apoptosis in H9c2 cardiomyoblasts during anoxia/reperfusion (A/R).

(a) siRNA knockdown effect by CST precursor (ChgA) protein expression. Immunofluorescence staining by cleaved caspase-3 for cell apoptosis (b) and western blot analysis of ER stress-marker expression (c). Cells with CST siRNA knockdown were treated with thapsigargin; immunohistochemistry of apoptosis and (d) western blot analysis of ER stress-markers expression (e). Five independent experiments were performed for these studies. Scale bar = 100 μM.