Table 1. Summary of the results of the comparative study of isolates 3D7 and FCR3 cultivated under different conditions.
| Isolate 3D7 |
Isolate FCR3 |
|||||||
|---|---|---|---|---|---|---|---|---|
| AlbuMAX |
Human Serum |
AlbuMAX |
Human Serum |
|||||
| K–* | K+† | K– | K+ | K– | K+ | K– | K+ | |
| Number of knobs/μm | — | 0.75 ± 0.09 | — | 1.24 ± 0.14 | — | 1.03 ± 0.09 | — | 1.27 ± 0.13 |
| Knobs surface area (nm2) | — | 6986 ± 4970 | — | 5940 ± 4211 | — | 10961 ± 6681 | — | 4433 ± 3356 |
| Diameter/height of knobs (nm) | — | 87 ± 38/13 ± 9 | — | 82 ± 27/4 ± 3 | — | 115 ± 40 | — | 7 ± 5 |
| Cytoadhesion (IE/100 CHO cells) | ||||||||
| CD36 | 44.3 ± 9.4 | 79.6 ± 15.3 | 250 ± 28.5 | 170 ± 13.9 | 76.9 ± 19.4 | 72.2 ± 17.8 | 261.2 ± 37.2 | 113.7 ± 12 |
| P-selectin | 0 | 0.2 ± 0.1 | 7.5 ± 3.4 | 0.2 ± 0.2 | 1.7 ± 0.4 | 2.7 ± 1 | 4.3 ± 1 | 0.7 ± 0.5 |
| E-selectin | 0 | 0.1 ± 0.1 | 0.7 ± 0.4 | 0.7 ± 0.4 | 0 | 0 | 1.2 ± 0.7 | 0.5 ± 0.3 |
| ICAM-1 | 0 | 0.3 ± 0.1 | 3.5 ± 1.3 | 28.9 ± 4.5 | 0.1 ± 0.1 | 15 ± 2.3 | 2.5 ± 0.7 | 11 ± 1.7 |
| CD9 | 0.1 ± 0.1 | 0.4 ± 0.1 | 5 ± 2.2 | 10 ± 2.2 | 0.2 ± 0.2 | 4.2 ± 1 | 3.7 ± 0.8 | 1.4 ± 0.3 |
| CD151 | 0.3 ± 0.1 | 0.2 ± 0.1 | 1.8 ± 1.4 | 2.3 ± 0.9 | 0.8 ± 0.4 | 0.4 ± 0.3 | 3 ± 1.3 | 0 |
| MDR1 | 0 | 0 | 1.7 ± 0.8 | 2.9 ± 1.6 | 0.1 ± 0.1 | 0.9 ± 0.4 | 1.6 ± 0.8 | 0.2 ± 0.2 |
| Localization (%), Negative/Parasit/MC/EM‡ | ||||||||
| RIFIN | 1/98/3/22 | 1/98/2/17 | 4/94/0/5 | 1/99/2/5 | 18/78/2/20 | 10/74/0/37 | 10/90/0/1 | 1/97/0/2 |
| STEVOR | 1/78/1/40 | 2/89/0/94 | 14/75/0/34 | 9/89/0/10 | 3/48/1/84 | 1/14/0/98 | 11/85/0/5 | 11/88/0/8 |
| PfMC-2TM | 0/63/0/75 | 1/66/0/91 | 0/95/0/33 | 8/82/0/40 | 21/47/0/43 | 16/39/0/51 | 2/85/0/23 | 6/80/0/45 |
| Differential gene expression (threshold ≥3 fold, padj <0.05) | K− > K+ = 28 genesK− < K+ = 8 genes | No differential expression | ||||||
| AlbuMAX_K− > Human Serum_K− = 873AlbuMAX_K+ > Human Serum_K+ = 496AlbuMAX_K− < Human Serum_K− = 8AlbuMAX_K+ < Human Serum_K+ = 24 | ||||||||
*K− (knobless infected erythrocytes).
†K+ (knobby infected erythrocytes).
‡Negative (without specific fluorescence signal), Parasite (inside parasitic bounderies), MC (Maurer’s clefts), EM (Erythrocyte membrane).