Figure 3. In vitro multilineage differentiation of PBMSCs.

(A,B) Calcium deposits, orange-red color stained by alizarin red or black color stained by von kossa staining, were scattered in the osteogenic induced culture. (C,D) ALP and osteocalcin expression in the osteogenic cells were illustrated by ALP staining and immunocytochemistry. (E) aggrecan expressed in chondrogenesis induced cells was demonstrated by Toluidine blue staining. (F) lipid vacuoles in the adipogenesis induced cells were showed by Oil Red O staining. (G,H) induced neurons and Schwann cells were identified by immunocytochemistry with antibodies of β-tubulin III and S100.