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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Biochim Biophys Acta. 2015 Apr 7;1852(7):1372–1379. doi: 10.1016/j.bbadis.2015.04.002

Fig. 1.

Fig. 1

Secretion of PNLIP and p.T221M PNLIP from transfected HEK 293A cells. The media from transfected cells was sampled 72 h after transfection. (A) The lipase activity against tributyrin in 50 μl of medium was measured in the standard 5 minute pH-stat assay. The mean and SD of 3 separate determinations is plotted. (B) The presence of PNLIP was determined by SDS PAGE and protein immunoblot with the rabbit anti-human PNLIP antisera followed by detection with the HRP conjugated goat anti-rabbit IgG antisera. (C) The relative amounts of lipase in the samples were determined by densitometry of 3 separate experiments.