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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Biochim Biophys Acta. 2015 Apr 7;1852(7):1372–1379. doi: 10.1016/j.bbadis.2015.04.002

Fig. 5.

Fig. 5

Effect of expression of p.T221M PNLIP in AR42J cells on ER stress. The cells were transduced with three different titers of each adenovirus construct. Total RNA was isolated from the cells as described. The amount of mRNA encoding BiP and calreticulin was determined by real-time RT-PCR and the amount of XBP1 mRNA splicing determined by RT-PCR, separation by agarose gel electrophoresis, ethidium bromide staining and densitometry. (A) Mean and SD of the relative BiP levels. (B) Mean and SD of the relative calreticulin levels. (C) Amount of XBP1 mRNA splicing is given. The upper panel shows a representative agarose gel separation of the RT-PCR products. The bottom panel presents the mean and SD of 3 experiments. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001.