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. 2015 Sep 23;89(23):12221–12225. doi: 10.1128/JVI.02382-15

FIG 3.

FIG 3

pUL93 is required for nucleocapsid maturation. (A to F) Transmission electron micrographs (TEM) of HF infected with WT-TB40/E (A, C, and E) or UL93st-TB40/E-BAC (UL93st) (B, D, and F) viruses at an MOI of 3.0 and fixed for processing at 4 days postinfection. (A and B) A single infected cell showing nuclear inclusion (NI) as well as cytoplasmic inclusion (CI). (C and D) Nuclear inclusion illustrating A- (black arrowhead), B- (black-tailed arrow), and C- (white-tailed arrow) capsids. (E and F) Cytoplasmic inclusion illustrating mature virions (white-tailed arrow) and dense bodies (white arrowhead). Bars, 4 μm (A and B), 500 nm (C and D), and 1 μm (E and F). (G to K) Magnified images (not to scale) of A-capsids (G), B-capsids (H), C-capsids (I), virions (J), and dense bodies (K) are shown. (L and M) A-, B-, and C-capsids in the nucleus (L) and virions, dense bodies, and nontypeable particles in the cytoplasm (M) were enumerated in 4 individual infected cells, and the mean values are displayed.