FIG 4.

pUL93 is required for viral genome cleavage. DNA extracted from HF infected with WT-TB40/E in the presence of BDCRB, infected with WT-TB40/E in the absence of BDCRB, or infected with UL93st-TB40/E-BAC virus (UL93st) at an MOI of 3.0 was digested with EcoRI or KpnI, size fractionated by gel electrophoresis, and blotted. Hybridization was performed using a terminal DNA probe. Fragments labeled 6 and 12 in the autoradiographic image are terminal fragments that represent cleaved viral genomic DNA. Fragments 1, 2, 3, 4, 5, 7, 8, 9, 10, and 11 are all possible junction fragments.