FIG 5.
T-cell-mediated immune response elicited by clones B141 and B388 in immunized mice. BALB/c mice were either mock immunized or immunized with 1 × 106 PFU of B141 or B388 via tail scarification, and spleens were removed 21 days postimmunization. (A) Detection of secreted IFN-γ by ELISA. Splenocytes from individual mice were plated separately (closed symbol) or were pooled by group before plating (open symbols). Data are presented as the mean IFN-γ concentration from 4 independent experiments (n = 3 mice per group per experiment). A lognormal distribution was assumed for all data sets. **, P < 0.01; ***, P < 0.001 (one-way ANOVA followed by Tukey's posttest). (B) Immunophenotyping of mouse splenocytes. Splenocytes were pooled, stimulated in vitro, and then stained with the indicated fluorochrome-conjugated antibodies, followed by flow cytometry analysis. Data from 2 independent experiments are presented as the mean percentages (±SEM) from CD4 (left) or CD8 cells (right) that express the indicated combinations of cytokines.