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SDS-PAGE analysis of purified recombinant SIVmac239 integrase proteins. Lane 1, molecular mass ladder; lanes 2 to 13, SIVmac239 IN proteins as labeled. About 2 to 3 μg of protein was loaded into lanes 2 to 13. After nickel affinity chromatography and dialysis, protein samples were analyzed on 10% polyacrylamide gels as outlined in Materials and Methods. The arrowhead indicates a molecular mass of ∼34 kDa, corresponding to that of the recombinant SIV protein.
