FIG 7.
Competitive inhibition of strand transfer by DTG, EVG, and RAL. Strand transfer reactions were carried out in the presence of 400 nM integrase proteins. Raw data from two independent experiments were fitted for the competitive inhibition equation using GraphPad Prism 5.0. (A) A representative plot for competitive inhibition of WT integrase in the presence of DTG is shown. (B, C, and D) The fold change susceptibility of each variant protein for DTG (B), EVG (C), and RAL (D) was calculated for each enzyme by dividing the Ki of the corresponding variant enzyme by that of the WT. Data represent the means of results from at least three independent experiments. Error bars represent standard errors of the means (SEM). Asterisks indicate statistically significant differences. Statistical significance was calculated for individual pairs of data using a one-sample two-tailed t test with a statistical cutoff of P ≤ 0.05.