FIG 2.
Negative-stain EM reference-free 2D class averages of 92UG037.8 and CZA97.012 SOSIP.664-His and gp140UNC-Fd-His proteins purified by different methods. (A) 92UG037.8 SOSIP.664-His trimers purified by 2G12/SEC and CZA97.012 SOSIP.664-His trimers purified by GNA-lectin/SEC; (B) 92UG037.8 SOSIP.664-His trimers purified by PGT145/SEC and CZA97.012 SOSIP.664-His trimers purified by PGT151/SEC; (C) 92UG037.8 and CZA97.012 gp140UNC-Fd-His proteins purified by Ni-NTA/SEC. The percentage of native-like trimers is listed below each set of class averages. Any discernible aggregates, dimers, and monomers (based on relative mass/size) are not included in the class averages or the calculations of the percentage of native-like trimers.