FIG 3.
SEC profiles of SOSIP.664-His and gp140UNC-Fd-His proteins. (A) PGT145-purified 92UG037.8 and PGT151-purified CZA97.012 SOSIP.664-His proteins; (B) Ni-NTA-purified 92UG037.8 and CZA97.012 gp140UNC-Fd-His proteins. In panel B, the arrows show when collection of the trimer fractions was initiated. (C) The trimer fractions shown in panel B were pooled and reanalyzed by SEC (ReSEC). In each panel, the x axis shows the fractions collected over time and the y axis shows absorbance at 280 nm. In each panel, the inset shows a BN-polyacrylamide gel with the most prominent bands indicated.