FIG 4.
SDS-PAGE analysis of SOSIP.664-His and gp140UNC-Fd-His proteins. (A and B) Reducing gels (i.e., with DTT [+ DTT]) versus nonreducing gels of PGT145/SEC-purified 92UG037.8 and PGT151/SEC-purified CZA97.012 SOSIP.664-His trimers (A) and Ni-NTA-purified 92UG037.8 and CZA97.012 gp140UNC-Fd-His proteins (B). The positions of gp120 and gp140 bands are indicated by the black lines or black arrow to the right of the gels. The positions of molecular mass markers (in kilodaltons) are indicated to the left of the gels.