FIG 5.
Protease sensitivity of SOSIP.664-His trimers and gp140UNC-Fd-His proteins. (A and B) 92UG037.8 (A) and CZA97.012 (B) proteins were incubated with the indicated concentrations (in nanograms per milliliter) of trypsin, chymotrypsin, or proteinase K for 1 h at 37°C and analyzed by reducing SDS-PAGE. The 92UG037.8 and CZA97.012 SOSIP.664-His trimers were purified by the PGT145/SEC and PGT151/SEC methods, respectively, and the two gp140UNC-Fd-His proteins were purified via Ni-NTA/SEC. In panels A and B, the 0-concentration lanes of the trypsin and chymotrypsin gels are duplicate depictions of the same control samples, as they are common to both of the protease titration experiments that were performed simultaneously and analyzed on the same gel.