FIG 6.
SPR analyses of SOSIP.664-His trimers and gp140UNC-Fd-His proteins. Association and dissociation profiles for the indicated MAbs are shown. CD4-IgG2 and all MAbs other than 2G12 at 500 nM flowed over His tag-immobilized Env proteins that were present on the chips in equivalent amounts (∼5% standard deviation [SD]). The 2G12 MAb was injected at 62 nM (see Materials and Methods). (A) 92UG037.8 proteins; (B) CZA97.012 proteins. Note that the y-axis scales differ among the plots. The Env proteins were purified as described in the legend to Fig. 5.