FIG 7.

Correlation between antigenicity of SOSIP.664 trimers and neutralization of Env-pseudotyped viruses. The plots for 92UG037.8 and CZA97.012 compare EC₅₀s for MAb binding derived from His tag capture ELISAs with IC₅₀s derived from TZM-bl cell neutralization assays using the same MAbs and the corresponding Env-pseudotyped viruses. Data on the MPER-specific bNAbs 2F5 and 4E10 were not included in these analyses, as these epitopes are not present on SOSIP.664-His trimers. (A) PGT145/SEC- or PGT151/SEC-purified SOSIP.664-His trimers; (B) Ni-NTA/SEC-purified gp140_UNC-Fd-His proteins. The MAb panel tested and the epitopes each MAb recognizes are listed in Table 1. In panel A, data points for multiple non-NAbs are overlapping and circled. Also highlighted is the anomalous binding of the 35O22 bNAb to the 92UG037.8 SOSIP.664-His trimer despite its limited ability to neutralize the corresponding virus (see the text). The data points plotted at MAb concentrations of 30,000 ng/ml in the ELISA or 10,000 ng/ml in the neutralization assay represent values that were endpoints that were not reached in these assays (see Table 1).