FIG 8.
Thermal stability of SOSIP.664-His and gp140UNC-Fd-His proteins. DSC profiles and derivative values are shown for PGT145/SEC-purified 92UG037.8 SOSIP.664-His trimers, PGT151/SEC-purified CZA97.012 SOSIP.664-His trimers, Ni-NTA/SEC-purified 92UG037.8 gp140UNC-Fd-His, and CZA97.012 gp140UNC-Fd-His proteins. For the SOSIP.664-His trimers, 40 μg was used in each experiment, and for the gp140UNC-Fd-His proteins, 60 μg was used. Plots of the raw data are shown in the top panels, and the baseline and molarity-corrected plots are shown in the bottom panels. Derivative values for the SOSIP.664-His trimers were determined by modeling a non-two-state curve over the baseline-corrected data. The corresponding values for the gp140UNC-Fd-His proteins could not be determined because clear peaks were not visible in the raw data. Temperature (in degrees Celsius) is shown on the x axes of the plots; the crossing point (Cp) (in millicalories or calories per degree) is shown on the y axes of the plots. T1/2, half-life, Tonset, time of onset.