TABLE 1.
MAb neutralization and Env-binding ELISA titers
| Epitope | Antibodya | 92UG037.8b |
CZA97.012b |
||||
|---|---|---|---|---|---|---|---|
| Neutralization IC50 (ng/ml) | ELISA binding EC50 (ng/ml) |
Neutralization IC50 (ng/ml) | ELISA binding EC50 (ng/ml) |
||||
| SOSIP.664-His trimer | gp140UNC-Fd-His | SOSIP.664-His trimer | gp140UNC-Fd-His | ||||
| CD4bs | CD4-IgG2 | 1,900 | 5,000 | 65 | 3,900 | 5,000 | 200 |
| VRC01 | 230 | 350 | 50 | 900 | 6,500 | 300 | |
| 3BNC60 | 40 | 1,100 | 180 | 400 | 6,000 | 5,000 | |
| CH103 | 12,300 | >10,000 | 550 | >30,000 | >10,000 | 300 | |
| b12 | >30,000 | >10,000 | 5,000 | >30,000 | >10,000 | 5,000 | |
| b6 | >30,000 | >10,000 | 400 | >30,000 | >10,000 | 110 | |
| F105 | >30,000 | >10,000 | 450 | >30,000 | >10,000 | 5,000 | |
| V1/V2-glycan | PG9 | 160 | 3,600 | >10,000 | >30,000 | >10,000 | >10,000 |
| PG16 | 30 | 550 | >10,000 | >30,000 | >10,000 | >10,000 | |
| PGT145 | 3,150 | 1,370 | >10,000 | 4,160 | >10,000 | >10,000 | |
| V3-glycan | PGT122 | 1,200 | 4,000 | 550 | 40 | 115 | 800 |
| PGT128 | 50 | 218 | 25 | 80 | 100 | 500 | |
| OD-glycan | 2G12 | >30,000 | 2,910 | 5,000 | >30,000 | >10,000 | >10,000 |
| PGT135 | >30,000 | >10,000 | 9,000 | >30,000 | >10,000 | >10,000 | |
| gp120-gp41 interface | PGT151 | >30,000 | >10,000 | >10,000 | 170 | 100 | >10,000 |
| 35022 | 30,000c | 55 | >10,000 | >30,000 | 1,000 | >10,000 | |
| 8ANC195 | 6,700 | 5,000 | >10,000 | >30,000 | >10,000 | >10,000 | |
| MPER | 2F5 | 3,024d | NA | >10,000 | >30,000d | NA | >10,000 |
| 4E10 | 8,840d | NA | >10,000 | 4,700d | NA | >10,000 | |
| V3 | 14e | >30,000 | >10,000 | 150 | >30,000 | >10,000 | 300 |
| 19b | >30,000 | >10,000 | 600 | >30,000 | >10,000 | >10,000 | |
| 39F | >30,000 | >10,000 | 520 | >30,000 | >10,000 | 1,000 | |
| CD4i | 17b | >30,000 | >10,000 | 9,000 | >30,000 | >10,000 | 1,600 |
| Cluster I (gp41) | F240 | >30,000 | >10,000 | 25 | >30,000 | >10,000 | 20 |
The test MAbs are grouped by epitope cluster.
The values shown in the table are 50% neutralization titers (IC50 [in nanograms/milliliter]) derived from Env-pseudotyped virus-based assays in Tzm-bl cells and midpoint binding titers (EC50 [in nanograms/milliliter]) from His tag ELISAs using the SOSIP.664-His and gp140UNC-Fd-His Env proteins. The > symbol in front of values indicates that endpoints were not achieved at MAb concentrations of 30,000 ng/ml in the neutralization assay or 10,000 ng/ml in the ELISA. NA, not applicable (SOSIP.664 trimers do not express epitopes for bNAbs against the MPER).
For bNAb 35O22 versus the 92UG037.8 virus, an IC50 could not be determined precisely because the extent of neutralization reached a plateau value at ∼50% (see the text).
Values for neutralization by MAbs 2F5 and 4E10 were derived from reference 87.