TABLE 2.

Glycan composition of 92UG037.8 and CZA97.012 SOSIP.664-His and gp140_UNC-Fd-His proteins

HIV-1 isolate or genotype and proteinb	Glycan composition (% of total glycans)a
	M5	M6	M7	M8	M9	Total of M5 to M9
CZA97.012
gp140UNC-Fd-His	10	4	7	9	7	37
SOSIP.664-His gp120	7	7	11	21	20	65
SOSIP.664-His gp41	5	5	4	1	0	16
92UG037.8
gp140UNC-Fd-His	10	3	6	13	13	46
SOSIP.664-His gp120	14	6	9	14	27	72
SOSIP.664-His gp41	6	4	3	0	0	13
BG505
WT.SEKS gp140*	6	5	8	12	11	42
SOSIP.664 gp120*	4	4	6	15	34	63
SOSIP.664 gp41*	6	9	12	5	2	34

^aThe data represent the abundance (as a percentage of total glycans) of the oligomannose structures (Man_5–9GlcNAc₂; indicated as M5 to M9), individually and in total, for each Env protein analyzed. The remaining glycans are of the complex and hybrid type, similar to what is described in more detail elsewhere for other, similar Env proteins (39). The 92UG037.8 and CZA97.012 gp140_UNC-Fd-His proteins were purified by Ni-NTA/SEC, and the values presented are for the entire gp140 protein (i.e., gp120 plus gp41_ECTO), as it was not possible to separate the individual subunits. The 92UG037.8 SOSIP.664-His trimers were purified by PGT145/SEC, and the CZA97.012 SOSIP.664-His trimers were purified by PGT151/SEC. For these trimers, values are also presented for the individual gp120 and gp41_ECTO subunits, which were separated for analysis on a reducing SDS-polyacrylamide gel.

^bFor comparison, data derived from BG505 Env proteins and published elsewhere (39) are also shown (the proteins are indicated by an asterisk). WT.SEKS is an uncleaved, nonnative gp140 protein similar to gp140_UNC-Fd-His but lacking the Fd and His domains (19). Both BG505 Env proteins were expressed in 293T cells and purified by 2G12/SEC.