FIG 5.

HCMV-infected cells induce mitochondrial biogenesis and mitochondrial morphological changes in the absence of Rb. (A) Serum-starved scramble control (Scr) or Rb knockdown cells (Rb) were mock infected or infected at an MOI of 1, and the ratio of mitochondrial DNA (16S) to cellular genomic DNA (GAPHD) was measured by quantitative PCR at 8 or 72 h postinfection. Bars represent the means ± the standard deviations of three biological replicates. Statistical significance was determined by using a Student t test. There was a statistically significant increase in the relative accumulation of mitochondrial DNA at 72 h compared to mock-infected cells in both the scrambled and Rb knockdown cells (P < 0.05); however, there was no statistical significance between the scrambled control and Rb knockdown cells at any time point (n.s., P > 0.3) (B to D) Representative images of serum-starved scrambled control (Scr) or Rb knockdown (Rb2) cells either mock infected (B) or infected at an MOI of 1 for 5 h (C) or 72 h (D) before staining with MitoTracker Red (red), pp71 (green), and Hoechst (blue). The green channel was omitted from the 72 hpi to aid in the visualization of the mitochondria.