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. 2015 Sep 16;89(23):11786–11800. doi: 10.1128/JVI.01356-15

FIG 1.

FIG 1

VSV-gp160G selectively infects and replicates within CD4+ cells. (A) Schematic representation of VSV-XN2 construct and VSV-gp160G construct with gp160G fusion protein replacing VSV-G. (B) Graphical representation of VSV-XN2 and VSV-gp160G virions depicting the replacement of VSV-G with an HIV gp160–VSV-G fusion. The relevant glycoproteins are VSV-G, HIV-gp160, and the HIV-gp160–VSV-G fusion protein gp160G (left, right, bottom). (C) Bright-field microscopy of HeLa and HeLa CD4+ cells infected with either VSV-XN2 or VSV-gp160G at an MOI of 0.01 at 24 hpi. (D) Immunoblot analysis results for viral glycoprotein expression of either construct in infected HeLa or HeLa CD4+ cells infected at an MOI of 0.01 at 24 hpi. (E) Growth kinetic assay results for HeLa or HeLa CD4+ cells infected with either VSV-XN2 or VSV-gp160G at an MOI of 0.001. Supernatant was analyzed by a standard plaque assay using HeLa CD4+ cells (Student t test, two tailed, equal variance; **, P < 0.001).