FIG 7.

Infection of PMH cultures with HCV Jad. (A) PMH cultures (gray bars) or Huh7.5 cells (white bars) were infected with Jad at an MOI of 0.04 TCID₅₀/cell, and viral production was determined at the indicated times p.i. by RT-qPCR titration of the culture supernatants. (B) PMH cultures were infected with untreated or UV-inactivated Jad at an MOI of 0.02 (gray bars) or 0.14 (black bars) TCID₅₀ per cell. Viral production was determined at 3 days p.i. by RT-qPCR titration of the culture supernatants. Mean results ± standard deviations from two experiments each performed in duplicates or triplicates are represented. (C) PMH cultures were incubated with Jad at a MOI of 0.14 TCID₅₀ per cell in the presence of anti-CD81 (hatched bar) or control isotype-matched (black bar) antibodies for 4 h and further incubated in the absence of antibodies for 72 h. Viral production was quantified at 72 h p.i. by RT-qPCR titration of the culture supernatants and expressed with respect to viral titers obtained in the presence of control antibodies set at 100%.